Vitrification of Oocytes and Embryos

Mouse Oocytes and Embryos Cryotop-vitrification Using Low Concentrated Solutions: Effects on Meiotic Spindle, Genetic Material Array and Developmental Ability

P-118: Developmental Competence of Ovine Embryos Derived from Frozen Oocytes Co-Cultured with Embryos Derived from Fresh Oocytes

Background: Currently, a limited number of studies have been done on the vitrification of small ruminant oocytes, especially in sheep, where poor developmental rates are obtained following immature and mature oocyte vitrification. Poor ovine oocyte cryopreservation could attribute to the damage to enzymes which has role in metabolic pathways. Therefore, production of cytokines and growth factor...

Cryopreservation of In Vitro Produced Embryos and Immature Oocytes in Pigs

Gamete cryopreservation combined with in vitro embryo production (IVP) and cryopreservation of resultant embryos are important technologies for gene banking in domestic animals. Especially, cryopreservation of embryos and oocytes offers the possibility for the preservation of female genetic lines. Although several vitrification protocols have been developed for the cryopreservation of embryos a...

Prospective randomized comparison of human oocyte cryopreservation with slow-rate freezing or vitrification.

OBJECTIVE To compare cryopreservation of mature human oocytes with slow-rate freezing and vitrification and determine which is most efficient at establishing a pregnancy. DESIGN Prospective randomized. SETTING Academically affiliated, private fertility center. PATIENT(S) Consenting patients with concerns about embryo cryopreservation and more than nine mature oocytes at retrieval were ran...

P-86: Determining The Most Oocyte Challenging Step during Vitrification-Warming in Sheep

Background: Oocyte cryopreservation is of utmost importance in the current repertories of assisted reproductive technology (ART). Despite this, oocyte vitrification is yet a problematic issue with limited successful pregnancies has been arisen after transfer of embryos derived from vitrified-warmed oocytes. In this sense, great attention has been paid to investigate possible mechanisms underlyi...

P-72: Ovine Oocytes Vitrified at Germinal Vesicle Stage as Cytoplast Recipients forSomatic Cell Nuclear Transfer (SCNT)

Background: The cryopreservation of immature oocytes at the germinal vesicle (GV) stage would create an easily accessible, nonseasonal source of female gametes for research and reproduction. The present study investigated the ability of ovine oocytes vitrified at the GV stage using a cryoloop to be subsequently matured, fertilized and cultured in vitro to blastocyst-stage embryos. Materials and...